competitive elisa  (Absolute Biotech Inc)

Journal: Journal of Bacteriology

Article Title: Synergistic interactions between glycogen and trehalose mediate adaptation to the stationary phase in E. coli

doi: 10.1128/jb.00544-25

Figure Lengend Snippet: Glycogen and trehalose synergistically buffer against glucose stress. ( A ) Glycogen and trehalose quantification of WT stationary phase cultures (16 h post-inoculation) in LB medium and LB medium supplemented with increasing glucose concentrations (0.1%, 0.2%, and 0.4%). Data are mean ± s.d.( n = 3 cultures per strain or per condition. Statistical significance of glucose-supplemented conditions is compared to LB-only media condition: *, P < 0.05; **, P <0.01 ; ***, P < 0.001; unpaired t -test; two-sided). ( B ) Growth curve of strains in LB + 0.4% glucose. Black filled squares, WT; dark gray open squares, otsA; gray circles, glgA ; light gray triangles, glgA otsA . (↓= below limit of detection, 10 3 CFU/mL) Statistically significant timepoints are indicated in the accompanying table: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant; unpaired t-test; two-sided. ( C ) AGE ELISA. Data are mean ± s.d. ( n = 3 cultures per strain or per condition; Statistical significance of AGE concentration of mutant strains is compared to AGE concentration of WT for the corresponding day/condition; *, P < 0.05; unpaired t -test; two-sided).

Article Snippet: 100 μL of protein extract, at concentrations ranging from 0.5 to 1.0 mg/mL, was added to an AGE conjugate-coated plate as part of an OxiSelect AGE Competitive ELISA Kit (Cell Biolabs).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Mutagenesis